Thank you for your letter. I've taken a look at your results and agree that there are likely two different haplogroups - although they are properly called Haplogroup R1b and Haplogroup I (the old haplogroups 1 and 2 respectively). Analysis cannot be made solely on the one marker to predict a haplogroup as these markers do of course change with time, so a comparison is made on many markers to give a clearer picture. To answer your question regarding relatedness within different haplogroups, no they would not be related. They will share common ancestors many thousands of years ago, but this will be before the defining SNP marker occurred. However, the test doesn't define haplogroups and thus a count of genetic distance has to be taken. The Sturtevant and Sturtivant tests actually differ by just three markers. The DYS461 alleles are the same for each individual - an announcement to Ybase and DNA Heritage customers was made regarding the DYS461 marker (and others) a couple of days ago but is only sent to people who have specifically signed up to the newsletters. Information regarding the marker changes has also been sent out with the reports and certificates. A genetic distance of three is quite high but, I would say yes, the two individuals are likely related. It's possible you can test someone between them and find a 'bridge' - i.e. someone with a haplotype in between both of them and possibly deduce where the mutation may have occurred. We don't place too much emphasis on the time to the MRCA (as in an estimation of when the MRCA actually lived (there are large errors)) although MRCA estimates are useful for comparing high and low-resolution tests. Your other participant is indeed (and quite conclusively) unrelated to the first two. This maybe just the one individual that is unrelated, or it may be the entire line. I would not recommend you base the entire line on solely one participant as there is of course the possibility that a non-paternity event has occurred, but obscured by only testing one individual. Projects do get support in the interpretation of the results and which people are best to test. Larger Projects may occasionally need network analysis too when they have many closely related individuals. Network analysis is best done solely with single-copy markers and only with markers that overlap, so this is a consideration if a larger project is envisaged. We are working hard to bring the new website pages up together which will hopefully help your Project along.